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Expression of PLVAP and AGP in relation to GBM malignancy. (A) Double immunostaining and <t>immunofluorescence</t> showing the expression of Ki67 around PLVAP‐positive vessels. The lower panel shows a comparison of Ki67 positivity around PLVAP‐positive and negative vessels. (B) IHC of PLVAP in normal brain and glioma grades 2–4. (C) Plasma AGP levels in normal adults and human GBM patients. (D) IHC of AGP in normal brain and glioma grades 2–4. (E) AGP leakage around vessels in untreated GBM cases. (F) Comparison of AGP immunostaining before and after Bev treatment. ** p < 0.01, **** p < 0.0001.
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Expression of PLVAP and AGP in relation to GBM malignancy. (A) Double immunostaining and <t>immunofluorescence</t> showing the expression of Ki67 around PLVAP‐positive vessels. The lower panel shows a comparison of Ki67 positivity around PLVAP‐positive and negative vessels. (B) IHC of PLVAP in normal brain and glioma grades 2–4. (C) Plasma AGP levels in normal adults and human GBM patients. (D) IHC of AGP in normal brain and glioma grades 2–4. (E) AGP leakage around vessels in untreated GBM cases. (F) Comparison of AGP immunostaining before and after Bev treatment. ** p < 0.01, **** p < 0.0001.
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Expression of PLVAP and AGP in relation to GBM malignancy. (A) Double immunostaining and <t>immunofluorescence</t> showing the expression of Ki67 around PLVAP‐positive vessels. The lower panel shows a comparison of Ki67 positivity around PLVAP‐positive and negative vessels. (B) IHC of PLVAP in normal brain and glioma grades 2–4. (C) Plasma AGP levels in normal adults and human GBM patients. (D) IHC of AGP in normal brain and glioma grades 2–4. (E) AGP leakage around vessels in untreated GBM cases. (F) Comparison of AGP immunostaining before and after Bev treatment. ** p < 0.01, **** p < 0.0001.
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Expression of PLVAP and AGP in relation to GBM malignancy. (A) Double immunostaining and <t>immunofluorescence</t> showing the expression of Ki67 around PLVAP‐positive vessels. The lower panel shows a comparison of Ki67 positivity around PLVAP‐positive and negative vessels. (B) IHC of PLVAP in normal brain and glioma grades 2–4. (C) Plasma AGP levels in normal adults and human GBM patients. (D) IHC of AGP in normal brain and glioma grades 2–4. (E) AGP leakage around vessels in untreated GBM cases. (F) Comparison of AGP immunostaining before and after Bev treatment. ** p < 0.01, **** p < 0.0001.
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Expression of PLVAP and AGP in relation to GBM malignancy. (A) Double immunostaining and <t>immunofluorescence</t> showing the expression of Ki67 around PLVAP‐positive vessels. The lower panel shows a comparison of Ki67 positivity around PLVAP‐positive and negative vessels. (B) IHC of PLVAP in normal brain and glioma grades 2–4. (C) Plasma AGP levels in normal adults and human GBM patients. (D) IHC of AGP in normal brain and glioma grades 2–4. (E) AGP leakage around vessels in untreated GBM cases. (F) Comparison of AGP immunostaining before and after Bev treatment. ** p < 0.01, **** p < 0.0001.
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Expression of PLVAP and AGP in relation to GBM malignancy. (A) Double immunostaining and immunofluorescence showing the expression of Ki67 around PLVAP‐positive vessels. The lower panel shows a comparison of Ki67 positivity around PLVAP‐positive and negative vessels. (B) IHC of PLVAP in normal brain and glioma grades 2–4. (C) Plasma AGP levels in normal adults and human GBM patients. (D) IHC of AGP in normal brain and glioma grades 2–4. (E) AGP leakage around vessels in untreated GBM cases. (F) Comparison of AGP immunostaining before and after Bev treatment. ** p < 0.01, **** p < 0.0001.

Journal: Cancer Science

Article Title: Abnormal Vessels Potentially Accelerate Glioblastoma Proliferation by Inducing the Protumor Activation of Macrophages

doi: 10.1111/cas.70014

Figure Lengend Snippet: Expression of PLVAP and AGP in relation to GBM malignancy. (A) Double immunostaining and immunofluorescence showing the expression of Ki67 around PLVAP‐positive vessels. The lower panel shows a comparison of Ki67 positivity around PLVAP‐positive and negative vessels. (B) IHC of PLVAP in normal brain and glioma grades 2–4. (C) Plasma AGP levels in normal adults and human GBM patients. (D) IHC of AGP in normal brain and glioma grades 2–4. (E) AGP leakage around vessels in untreated GBM cases. (F) Comparison of AGP immunostaining before and after Bev treatment. ** p < 0.01, **** p < 0.0001.

Article Snippet: The following second antibodies were used for immunofluorescence staining: goat anti‐rabbit fluorescence conjugated secondary antibody (Alexa Fluor 594, Thermo Fisher Scientific, MA, USA) and goat anti‐mouse fluorescence‐conjugated secondary antibody (Alexa Fluor 488, Thermo Fisher Scientific).

Techniques: Expressing, Double Immunostaining, Immunofluorescence, Comparison, Clinical Proteomics, Immunostaining